Genotyping of Tunisian hepatitis B virus isolates based on the sequencing of preS2 and S regions.
Identifieur interne : 000003 ( Main/Exploration ); précédent : 000002; suivant : 000004Genotyping of Tunisian hepatitis B virus isolates based on the sequencing of preS2 and S regions.
Auteurs : I. Borchani-Chabchoub [Tunisie] ; A. Gargouri ; R. Mokdad-GargouriSource :
- Microbes and infection [ 1286-4579 ] ; 2000.
Descripteurs français
- KwdFr :
- ADN viral (sang), Analyse de séquence d'ADN (MeSH), Antigènes de surface du virus de l'hépatite B (composition chimique), Antigènes de surface du virus de l'hépatite B (génétique), Données de séquences moléculaires (MeSH), Génotype (MeSH), Humains (MeSH), Hépatite B chronique (virologie), Protéines de l'enveloppe virale (composition chimique), Protéines de l'enveloppe virale (génétique), Précurseurs de protéines (composition chimique), Précurseurs de protéines (génétique), Réaction de polymérisation en chaîne (méthodes), Séquence d'acides aminés (MeSH), Tunisie (MeSH), Virus de l'hépatite B (classification), Virus de l'hépatite B (génétique).
- MESH :
- composition chimique : Antigènes de surface du virus de l'hépatite B, Protéines de l'enveloppe virale, Précurseurs de protéines, Virus de l'hépatite B.
- génétique : Antigènes de surface du virus de l'hépatite B, Protéines de l'enveloppe virale, Précurseurs de protéines, Virus de l'hépatite B.
- méthodes : Réaction de polymérisation en chaîne.
- sang : ADN viral.
- virologie : Hépatite B chronique.
- Analyse de séquence d'ADN, Données de séquences moléculaires, Génotype, Humains, Séquence d'acides aminés, Tunisie.
- Wicri :
- geographic : Tunisie.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), DNA, Viral (blood), Genotype (MeSH), Hepatitis B Surface Antigens (chemistry), Hepatitis B Surface Antigens (genetics), Hepatitis B virus (classification), Hepatitis B virus (genetics), Hepatitis B, Chronic (virology), Humans (MeSH), Molecular Sequence Data (MeSH), Polymerase Chain Reaction (methods), Protein Precursors (chemistry), Protein Precursors (genetics), Sequence Analysis, DNA (MeSH), Tunisia (MeSH), Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics).
- MESH :
- chemical , blood : DNA, Viral.
- chemical , chemistry : Hepatitis B Surface Antigens, Protein Precursors, Viral Envelope Proteins.
- chemical , genetics : Hepatitis B Surface Antigens, Protein Precursors, Viral Envelope Proteins.
- geographic : Tunisia.
- classification : Hepatitis B virus.
- genetics : Hepatitis B virus.
- methods : Polymerase Chain Reaction.
- virology : Hepatitis B, Chronic.
- Amino Acid Sequence, Genotype, Humans, Molecular Sequence Data, Sequence Analysis, DNA.
Abstract
The S nucleotide sequences of five hepatitis B virus strains isolated from plasma samples of Tunisian patients with chronic hepatitis B were determined; the preS2 region of three of them were sequenced. According to the comparative analysis of S peptide sequences with the reported sequences in the database bank, the five hepatitis B strains were shown to be related to the D genotypic group, subtype ayw. The nature of residues at positions 125 and 127 allowed us to distinguish between each subtype of the D group and to class all five Tunisian sequences in the 'ayw2' subtype. Moreover, two of them (1366 and 523) contained a substitution of the invariant Cys69 by Arg and Cys221 by Phe, respectively. Potential structural modifications due to the Cys-Arg change are discussed.
DOI: 10.1016/s1286-4579(00)00365-8
PubMed: 10884611
Affiliations:
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Le document en format XML
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Borchani-Chabchoub</name><affiliation wicri:level="1"><nlm:affiliation>Centre de Biotechnologie de Sfax, BP'K', 3038, Sfax, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Centre de Biotechnologie de Sfax, BP'K', 3038, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Gargouri, A" sort="Gargouri, A" uniqKey="Gargouri A" first="A" last="Gargouri">A. Gargouri</name></author><author><name sortKey="Mokdad Gargouri, R" sort="Mokdad Gargouri, R" uniqKey="Mokdad Gargouri R" first="R" last="Mokdad-Gargouri">R. Mokdad-Gargouri</name></author></analytic><series><title level="j">Microbes and infection</title><idno type="ISSN">1286-4579</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>DNA, Viral (blood)</term><term>Genotype (MeSH)</term><term>Hepatitis B Surface Antigens (chemistry)</term><term>Hepatitis B Surface Antigens (genetics)</term><term>Hepatitis B virus (classification)</term><term>Hepatitis B virus (genetics)</term><term>Hepatitis B, Chronic (virology)</term><term>Humans (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Polymerase Chain Reaction (methods)</term><term>Protein Precursors (chemistry)</term><term>Protein Precursors (genetics)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Tunisia (MeSH)</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (sang)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Antigènes de surface du virus de l'hépatite B (composition chimique)</term><term>Antigènes de surface du virus de l'hépatite B (génétique)</term><term>Données de séquences moléculaires (MeSH)</term><term>Génotype (MeSH)</term><term>Humains (MeSH)</term><term>Hépatite B chronique (virologie)</term><term>Protéines de l'enveloppe virale (composition chimique)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Précurseurs de protéines (composition chimique)</term><term>Précurseurs de protéines (génétique)</term><term>Réaction de polymérisation en chaîne (méthodes)</term><term>Séquence d'acides aminés (MeSH)</term><term>Tunisie (MeSH)</term><term>Virus de l'hépatite B (classification)</term><term>Virus de l'hépatite B (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>DNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Hepatitis B Surface Antigens</term><term>Protein Precursors</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Hepatitis B Surface Antigens</term><term>Protein Precursors</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Tunisia</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Hepatitis B virus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Antigènes de surface du virus de l'hépatite B</term><term>Protéines de l'enveloppe virale</term><term>Précurseurs de protéines</term><term>Virus de l'hépatite B</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Hepatitis B virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes de surface du virus de l'hépatite B</term><term>Protéines de l'enveloppe virale</term><term>Précurseurs de protéines</term><term>Virus de l'hépatite B</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Réaction de polymérisation en chaîne</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>ADN viral</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Hépatite B chronique</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Hepatitis B, Chronic</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Genotype</term><term>Humans</term><term>Molecular Sequence Data</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Données de séquences moléculaires</term><term>Génotype</term><term>Humains</term><term>Séquence d'acides aminés</term><term>Tunisie</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Tunisie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The S nucleotide sequences of five hepatitis B virus strains isolated from plasma samples of Tunisian patients with chronic hepatitis B were determined; the preS2 region of three of them were sequenced. According to the comparative analysis of S peptide sequences with the reported sequences in the database bank, the five hepatitis B strains were shown to be related to the D genotypic group, subtype ayw. The nature of residues at positions 125 and 127 allowed us to distinguish between each subtype of the D group and to class all five Tunisian sequences in the 'ayw2' subtype. Moreover, two of them (1366 and 523) contained a substitution of the invariant Cys69 by Arg and Cys221 by Phe, respectively. Potential structural modifications due to the Cys-Arg change are discussed.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country></list><tree><noCountry><name sortKey="Gargouri, A" sort="Gargouri, A" uniqKey="Gargouri A" first="A" last="Gargouri">A. Gargouri</name><name sortKey="Mokdad Gargouri, R" sort="Mokdad Gargouri, R" uniqKey="Mokdad Gargouri R" first="R" last="Mokdad-Gargouri">R. Mokdad-Gargouri</name></noCountry><country name="Tunisie"><noRegion><name sortKey="Borchani Chabchoub, I" sort="Borchani Chabchoub, I" uniqKey="Borchani Chabchoub I" first="I" last="Borchani-Chabchoub">I. Borchani-Chabchoub</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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